Device

Part:BBa_K392040

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)


endoglucanase/CBM secretion testing construct for E. coli

Device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; E. coli PelB export tag attached upstream to enable secretion of protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 331
    Illegal NotI site found at 1475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1388
    Illegal BamHI site found at 524
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 666
    Illegal NgoMIV site found at 1591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 570


[edit]
Categories
Parameters
None